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human breast cancer cell line mcf 7  (ATCC)


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    ATCC human breast cancer cell line mcf 7
    Human Breast Cancer Cell Line Mcf 7, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 34713 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcf7+cells/MCF7/pmc13101642-243-7-13
    Average 99 stars, based on 34713 article reviews
    human breast cancer cell line mcf 7 - by Bioz Stars, 2026-09
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    Cell Culture:

    Article Title: Visible neural network framework
    Article Snippet: .. A549, A427, and MCF7 cells were retrieved from the American Type Culture Collection (ATCC) and cultured in DMEM: 10% FBS or EMEM+10% FBS according to ATCC recommendations. ..

    Article Title: Constant cGAS activation blunts STING signaling in DU145 prostate cancer cell line.
    Article Snippet: .. Cell culture. hTERT-BJ-5ta cells (human skin fibroblast cells, CRL-4001; American Type Culture Collection [ATCC]), MCF7 cells (human breast adenocarcinoma cells, HTB-22; ATCC), U2OS cells (human osteosarcoma cells; gift from T. Hirota), A549 cells (human lung adenocarcinoma cells, CCL-185; ATCC), DU145 cells (human prostate carcinoma cells, HTB-81; ATCC), HCT116 cells (human colorectal carcinoma cells, CCL-247; ATCC), HCT15 cells and DLD-1 cells (human colorectal carcinoma cells, TKG 0504 and TKG0379; Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Miyagi, Japan) were grown at 37°C in a 5% CO2 atmosphere in DMEM (Nacalai Tescue), supplemented with 10% fetal bovine serum (FBS, Serana) and penicillin/streptomycin Mixed Solution (Nacalai Tescue). ..

    Article Title: T-cell Receptor (TCR) targeting with Multivalent T-cell Engagers
    Article Snippet: .. A431, MDA-MB-231, and MCF7 cells were purchased from the Americal Type Cell Culture Collection (ATCC). ..

    Multiple Displacement Amplification:

    Article Title: Dpep, a Cell-Penetrating Peptide Targeting ATF5, CEBPB and CEBPD, Synergistically Combines with ABT-263 and Decitabine to Inhibit Cancer Cell Growth and Overcome Dpep Resistance
    Article Snippet: .. T98G, A375-ATCC, HCT116, MDA-MB-231 and MCF7 cells were obtained from and authenticated by the ATCC and routinely maintained in DMEM supplemented with 10% FBS and 100 U/mL Penicillin–Streptomycin at 37 °C in 5% CO 2 . .. These lines were examined using Universal Mycoplasma Detection Kit #30-1012K (ATCC, Manassas, VA, USA) and verified to be free from mycoplasma contamination.

    Article Title: T-cell Receptor (TCR) targeting with Multivalent T-cell Engagers
    Article Snippet: .. A431, MDA-MB-231, and MCF7 cells were purchased from the Americal Type Cell Culture Collection (ATCC). ..



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    Efficiency of XBP1 gene silencing in MCF7 and MDA-MB-231 cells. XBP1 expression was evaluated in MCF7 and MDA-MB-231 cells at the mRNA and protein levels by qPCR and western blot analysis, respectively. Protein expression was assessed following shRNA-2-mediated silencing. Data are presented as mean ± SEM from three independent experiments ( n = 3). Statistical analysis of qPCR data was performed using one-way ANOVA, whereas western blot data were analyzed using Student’s t-test. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001

    Journal: 3 Biotech

    Article Title: XBP1 gene silencing augments quercetin-induced apoptosis and anti-metastatic activity in breast cancer cell lines

    doi: 10.1007/s13205-026-04923-8

    Figure Lengend Snippet: Efficiency of XBP1 gene silencing in MCF7 and MDA-MB-231 cells. XBP1 expression was evaluated in MCF7 and MDA-MB-231 cells at the mRNA and protein levels by qPCR and western blot analysis, respectively. Protein expression was assessed following shRNA-2-mediated silencing. Data are presented as mean ± SEM from three independent experiments ( n = 3). Statistical analysis of qPCR data was performed using one-way ANOVA, whereas western blot data were analyzed using Student’s t-test. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001

    Article Snippet: Human breast cancer cell lines MCF7 (ATCC ® HTB-22TM) and MDA-MB-231 (ATCC ® HTB-26TM) were purchased from the American Type Culture Collection (ATCC, USA).

    Techniques: Expressing, Western Blot, shRNA

    Representative microscopic images of transduced cells. Upper panels: MCF7; lower panels: MDA-MB-231. ( A ) Brightfield and ( B ) TurboGFP fluorescence images demonstrating transduction in the indicated cell lines. Images are representative of three independent experiments with similar results and were captured at 10X magnification, scale bars: 200 μm

    Journal: 3 Biotech

    Article Title: XBP1 gene silencing augments quercetin-induced apoptosis and anti-metastatic activity in breast cancer cell lines

    doi: 10.1007/s13205-026-04923-8

    Figure Lengend Snippet: Representative microscopic images of transduced cells. Upper panels: MCF7; lower panels: MDA-MB-231. ( A ) Brightfield and ( B ) TurboGFP fluorescence images demonstrating transduction in the indicated cell lines. Images are representative of three independent experiments with similar results and were captured at 10X magnification, scale bars: 200 μm

    Article Snippet: Human breast cancer cell lines MCF7 (ATCC ® HTB-22TM) and MDA-MB-231 (ATCC ® HTB-26TM) were purchased from the American Type Culture Collection (ATCC, USA).

    Techniques: Fluorescence, Transduction

    Que-induced cytotoxicity and the combined effects of Que and XBP1 silencing on breast cancer cells viability. ( A , B ) Cell viability of MCF7 and MDA-MB-231 cells after treatment with increasing concentrations of Que (0–200 µM) for 24, 48, and 72 h. ( C ) Cell viability of MCF7 cells in the Control, Que, XBP1(-), and XBP1(-) + Que groups at 24, 48, and 72 h, using Que at its IC 50 concentration (154.262 µM). ( D ) Cell viability of MDA-MB-231 cells in the Control, Que, XBP1(-), and XBP1(-) + Que groups at 24, 48, and 72 h, using Que at its IC 50 concentration (192.103 µM). Data are presented as mean ± SEM from three independent experiments ( n = 3). Statistical analysis of cell viability was performed using one-way ANOVA followed by Tukey’s post-hoc test. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001

    Journal: 3 Biotech

    Article Title: XBP1 gene silencing augments quercetin-induced apoptosis and anti-metastatic activity in breast cancer cell lines

    doi: 10.1007/s13205-026-04923-8

    Figure Lengend Snippet: Que-induced cytotoxicity and the combined effects of Que and XBP1 silencing on breast cancer cells viability. ( A , B ) Cell viability of MCF7 and MDA-MB-231 cells after treatment with increasing concentrations of Que (0–200 µM) for 24, 48, and 72 h. ( C ) Cell viability of MCF7 cells in the Control, Que, XBP1(-), and XBP1(-) + Que groups at 24, 48, and 72 h, using Que at its IC 50 concentration (154.262 µM). ( D ) Cell viability of MDA-MB-231 cells in the Control, Que, XBP1(-), and XBP1(-) + Que groups at 24, 48, and 72 h, using Que at its IC 50 concentration (192.103 µM). Data are presented as mean ± SEM from three independent experiments ( n = 3). Statistical analysis of cell viability was performed using one-way ANOVA followed by Tukey’s post-hoc test. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001

    Article Snippet: Human breast cancer cell lines MCF7 (ATCC ® HTB-22TM) and MDA-MB-231 (ATCC ® HTB-26TM) were purchased from the American Type Culture Collection (ATCC, USA).

    Techniques: Control, Concentration Assay

    Colony formation capacity of MCF7 and MDA-MB-231 cells following Que treatment and XBP1 silencing. Representative colony images and quantitative colony counts are shown for MCF7 and MDA-MB-231 cells in the Control, Que, XBP1(-), and XBP1(-) + Que groups. Que was used at the IC 50 concentration determined for each cell line. Colonies were counted manually. Data are presented as mean ± SEM from three independent experiments ( n = 3). Statistical analysis was performed using one-way ANOVA followed by Tukey’s post-hoc test. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001

    Journal: 3 Biotech

    Article Title: XBP1 gene silencing augments quercetin-induced apoptosis and anti-metastatic activity in breast cancer cell lines

    doi: 10.1007/s13205-026-04923-8

    Figure Lengend Snippet: Colony formation capacity of MCF7 and MDA-MB-231 cells following Que treatment and XBP1 silencing. Representative colony images and quantitative colony counts are shown for MCF7 and MDA-MB-231 cells in the Control, Que, XBP1(-), and XBP1(-) + Que groups. Que was used at the IC 50 concentration determined for each cell line. Colonies were counted manually. Data are presented as mean ± SEM from three independent experiments ( n = 3). Statistical analysis was performed using one-way ANOVA followed by Tukey’s post-hoc test. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001

    Article Snippet: Human breast cancer cell lines MCF7 (ATCC ® HTB-22TM) and MDA-MB-231 (ATCC ® HTB-26TM) were purchased from the American Type Culture Collection (ATCC, USA).

    Techniques: Control, Concentration Assay

    Effects of Que, XBP1 silencing, and their combination on expression levels of apoptosis-associated genes in MCF7 and MDA-MB-231 breast cancer cell lines. ( A ) Relative mRNA expression levels of BAX, BCL2, CASP3, CASP7, CASP8, CASP9, CYCS, and FADD were analyzed using qPCR in the Control, Que, XBP1(-), and XBP1(-) + Que groups. ( B ) Protein level expressions of CASP3, CASP8, and CASP9 were evaluated by western blot analysis in the same treatment groups. Bar graphs in panel B represent densitometric quantification of western blot bands performed using ImageJ and normalized to GAPDH. Que was applied at the IC 50 concentration determined for each cell line. Data are presented as mean ± SEM from three independent experiments ( n = 3). Statistical analysis was performed using one-way ANOVA. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001

    Journal: 3 Biotech

    Article Title: XBP1 gene silencing augments quercetin-induced apoptosis and anti-metastatic activity in breast cancer cell lines

    doi: 10.1007/s13205-026-04923-8

    Figure Lengend Snippet: Effects of Que, XBP1 silencing, and their combination on expression levels of apoptosis-associated genes in MCF7 and MDA-MB-231 breast cancer cell lines. ( A ) Relative mRNA expression levels of BAX, BCL2, CASP3, CASP7, CASP8, CASP9, CYCS, and FADD were analyzed using qPCR in the Control, Que, XBP1(-), and XBP1(-) + Que groups. ( B ) Protein level expressions of CASP3, CASP8, and CASP9 were evaluated by western blot analysis in the same treatment groups. Bar graphs in panel B represent densitometric quantification of western blot bands performed using ImageJ and normalized to GAPDH. Que was applied at the IC 50 concentration determined for each cell line. Data are presented as mean ± SEM from three independent experiments ( n = 3). Statistical analysis was performed using one-way ANOVA. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001

    Article Snippet: Human breast cancer cell lines MCF7 (ATCC ® HTB-22TM) and MDA-MB-231 (ATCC ® HTB-26TM) were purchased from the American Type Culture Collection (ATCC, USA).

    Techniques: Expressing, Control, Western Blot, Concentration Assay

    Effects of Que, XBP1 silencing, and their combination on EMT- and metastasis-associated genes in MCF7 and MDA-MB-231 breast cancer cell lines. ( A ) Relative mRNA expression levels of EMT/metastasis-associated markers were analyzed by qPCR in the Control, Que, XBP1(-), and XBP1(-) + Que groups. ( B ) Protein level expressions of CDH1 and CDH2 were evaluated by western blot analysis in the same treatment groups. Bar graphs in panel B represent densitometric quantification of western blot bands performed using ImageJ and normalized to GAPDH. Que was applied at the IC 50 concentration determined for each cell line. Data are presented as mean ± SEM from three independent experiments ( n = 3). Statistical analysis was performed using one-way ANOVA. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001

    Journal: 3 Biotech

    Article Title: XBP1 gene silencing augments quercetin-induced apoptosis and anti-metastatic activity in breast cancer cell lines

    doi: 10.1007/s13205-026-04923-8

    Figure Lengend Snippet: Effects of Que, XBP1 silencing, and their combination on EMT- and metastasis-associated genes in MCF7 and MDA-MB-231 breast cancer cell lines. ( A ) Relative mRNA expression levels of EMT/metastasis-associated markers were analyzed by qPCR in the Control, Que, XBP1(-), and XBP1(-) + Que groups. ( B ) Protein level expressions of CDH1 and CDH2 were evaluated by western blot analysis in the same treatment groups. Bar graphs in panel B represent densitometric quantification of western blot bands performed using ImageJ and normalized to GAPDH. Que was applied at the IC 50 concentration determined for each cell line. Data are presented as mean ± SEM from three independent experiments ( n = 3). Statistical analysis was performed using one-way ANOVA. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001

    Article Snippet: Human breast cancer cell lines MCF7 (ATCC ® HTB-22TM) and MDA-MB-231 (ATCC ® HTB-26TM) were purchased from the American Type Culture Collection (ATCC, USA).

    Techniques: Expressing, Control, Western Blot, Concentration Assay

    Proposed mechanistic overview of the combined treatment with Que and XBP1 silencing in MCF7 and MDA-MB-231 breast cancer cells. The schematic summarizes the treatment-associated reduction in cell viability, suppression of colony formation, modulation of apoptosis-associated markers, and EMT-related molecular changes observed in the present study. The relationships shown are presented as a conceptual summary of the current findings

    Journal: 3 Biotech

    Article Title: XBP1 gene silencing augments quercetin-induced apoptosis and anti-metastatic activity in breast cancer cell lines

    doi: 10.1007/s13205-026-04923-8

    Figure Lengend Snippet: Proposed mechanistic overview of the combined treatment with Que and XBP1 silencing in MCF7 and MDA-MB-231 breast cancer cells. The schematic summarizes the treatment-associated reduction in cell viability, suppression of colony formation, modulation of apoptosis-associated markers, and EMT-related molecular changes observed in the present study. The relationships shown are presented as a conceptual summary of the current findings

    Article Snippet: Human breast cancer cell lines MCF7 (ATCC ® HTB-22TM) and MDA-MB-231 (ATCC ® HTB-26TM) were purchased from the American Type Culture Collection (ATCC, USA).

    Techniques: